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PerkinElmer - ALSU-TMEK1-A-HV - AlphaLISA Surefire Ultra Total MEK1 Detection Kit, 100 Assay Points

  • MFR SKU: ALSU-TMEK1-A-HV
  • ITEM #: 10207089
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ITEM #: 10207089
The AlphaLISA SureFire Ultra Total MEK1 assay is a sandwich immunoassay for quantitative detection of MEK1 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume. The 500 point kit contains reagents to run 500 wells in 384-well format, using a 20 µL reaction volume....
ITEM #: 10207089
The AlphaLISA SureFire Ultra Total MEK1 assay is a sandwich immunoassay for quantitative detection of MEK1 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume. The 500 point kit contains reagents to run 500 wells in 384-well format, using a 20 µL reaction volume. The 10, 000 point kit contains reagents to run 10, 000 wells in 384-well format, using a 20 µL reaction volume. The 50, 000 point kit contains reagents to run 500 wells in 384-well format, using a 20 µL reaction volume. In the AlphaLISA SureFire Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal.  The amount of light emission is directly proportional to the amount of protein present in the sample. AlphaLISA SureFire Ultra kits are compatible with: Cell and tissue lysates Antibody modulators Biotherapeutic antibodies Alpha SureFire kits can be used for: Cellular kinase assays Receptor activation studies Screening
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PerkinElmer - ALSU-TMEK1-A-HV - AlphaLISA Surefire Ultra Total MEK1 Detection Kit, 100 Assay Points

  • Item #: 10207089
  • MFR SKU: ALSU-TMEK1-A-HV
  • Assay Pathway: MAPK Assay Target: MEK1 Assay Target Class: Protein Detection Method: Alpha Experimental Type: In vitro Format: Microplates Product Brand Name: AlphaLISA SureFire Ultra Shipping Condition: Blue Ice Unit Size: 100
  • Assay Points

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