G-Biosciences - 786-285 - Copper Chelating Resin

  • Item #: 10049226
  • MFR SKU: 786-285
$128.95
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Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath et al (1), is based on the interaction of certain protein residues (histidines, cysteines, and to some e x tent tryptophans) with cations of transition metals. The Copper Chelating Resin is specifically designed for the purification of proteins that associate with copper ions (2-3), including 6 x histidine tagged proteins. Immobilized metal affinity chromatography (IMAC) resin utilizingcopper (Cu 2+ ) for the purification...
Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath et al (1), is based on the interaction of certain protein residues (histidines, cysteines, and to some e x tent tryptophans) with cations of transition metals. The Copper Chelating Resin is specifically designed for the purification of proteins that associate with copper ions (2-3), including 6 x histidine tagged proteins. Immobilized metal affinity chromatography (IMAC) resin utilizingcopper (Cu 2+ ) for the purification of 6 x histidine tagged proteins. This resin binds to si x histidine residues (6 x His), a common tag used in protein purification. The resin consists of iminodiacetate coupled to 6% cross-linked agarose beads. The iminodiacetate binds divalentcopper ion with a capacity of 20-40μmoles Cu 2+ /ml resin. The protein binding capacity is >50mg protein per ml resin. We have demonstrated binding of >100mg of a 50kDa 6XHis tagged proteins to a ml of resin. Immobilized Nickel , Cobalt and Zinc Chelating Resins are also available. Cobalt has the highest selectivity followed by Zinc, Nickel then Copper, but has the lowest loading capacity. Copper has the highest loading capacity, followed by Nickel then Zinc. Specific binding/wash and elution buffers are available. Click here to request bulk or custom sizes .
Features
  • For the purification of Copper Binding proteins, including 6 x His proteins
  • High capacity: >50mg/ml
  • Ligand density: 20-40μmoles Cu 2+ /ml resin
  • Bead Structure: 6% cross-linked agarose

Applications
  • Affinity purification of copper binding proteins
  • Affinity purification of proteins with a 6 x His tag.

Copper Chelating Resin, 10mL Resin
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G-Biosciences - 786-285 - Copper Chelating Resin

  • Item #: 10049226
  • MFR SKU: 786-285

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