G-Biosciences - 786-219 - FOCUS Extraction Buffer V, For 50mL

  • Item #: 10047757
  • MFR SKU: 786-219
$159.95
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Chaotropic e x traction buffers that preserve the native charge of proteins making them s uitable for sample e x traction for 2D gels
One of the most important considerations before running 2D gel electrophoresis is the choice of protein solubilization buffers. A suitable buffer must solubilize proteins effectively, without disturbing the native charge of the proteins.
Urea, a common chaotrope, is widely used for solubilization and denaturation of proteins. One of the disadvantages...
Chaotropic e x traction buffers that preserve the native charge of proteins making them s uitable for sample e x traction for 2D gels
One of the most important considerations before running 2D gel electrophoresis is the choice of protein solubilization buffers. A suitable buffer must solubilize proteins effectively, without disturbing the native charge of the proteins.
Urea, a common chaotrope, is widely used for solubilization and denaturation of proteins. One of the disadvantages of using urea is carbamylation. In water, urea e x ists in equilibrium with ammonium cyanate, the level of which increases with increasing temperature and pH. Cyanate reacts with α-amino and ε-amino groups of proteins and induces a change in the isoelectric point of proteins. This leads to artifactual results. Therefore, carbamylation must be avoided.
One way to minimize the risk of carbamylation is to prepare the urea-based reagents fresh before each use. G-Biosciences has developed a series of dry, urea-based, pre-mi x ed and ready-to-use solubilization buffers. Simply add an appropriate volume of the supplied rehydration buffer to the dry buffer. Then use to solubilize proteins, saving time and improving the quality of IEF/2D gel electrophoresis (Patent Pending).
FOCUS™ E x traction Buffers are also designed to be used as rehydration buffers for IPG strips.
FOCUS™ E x traction Buffers are e x perimentally optimized concentrations of critical, buffering and stabilizing agents, including urea, thiourea, Nonidet® P-40, CHAPS and sulfobetaines. The FOCUS™ E x traction Buffers are designed to produce optimal protein e x traction and improved spot resolution for 2D gel analysis.
A range of FOCUS™ E x traction Buffers have been developed. Depending on the nature of the samples, one or more of the buffers suitable for your applications can be ordered. FOCUS™ E x traction Buffer-I is suitable for most applications; however, for stronger solubilization effects, we recommend FOCUS™ E x traction Buffer-II, -III, -IV, -V or -VI. A trial kit that contains all si x FOCUS™ E x traction Buffers and each of their corresponding diluents is also available. The trial kit is designed for those who prefer to e x periment for establishing a suitable e x traction and solubilization buffer for their protein samples.
The following FOCUS™ E x traction Buffers, listed with their major components, are available:
Major Components
FOCUS ™ E x traction Buffer I Urea & Nonidet® P-40
FOCUS ™ E x traction Buffer II Urea & CHAPS
FOCUS ™ E x traction Buffer III Urea, thiourea, CHAPS & amidosulfobetaine (ASB)-16
FOCUS ™ E x traction Buffer IV Urea, thiourea, CHAPS & sulfobetaine (SB) 3-10
FOCUS ™ E x traction Buffer V Urea, thiourea & CHAPS
FOCUS ™ E x traction Buffer VI Urea, thiourea, CHAPS & non detergent sulfobetaine 201
For analysis of a proteome, a single buffer may not be suitable and sequential solubilization using different FOCUS™ E x traction Buffers will help in identifying more and new proteins (see figure 1). See the application note in the technical section below for more information.
Features
  • Convenient & easy to use: simply hydrate and use
  • Prevents urea-induced protein carbamylation
  • Prevents waste of unused reagents
  • No artifactual protein bands due to dust & human skin contamination

Applications
  • Optimal protein e x traction & solubilization for 2D gel electrophoresis & other applications
  • Rehydration buffer for IPG-strips
  • Improved spot resolution for 2D gel analysis

FOCUS™ Extraction Buffer V, For 50mL
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G-Biosciences - 786-219 - FOCUS Extraction Buffer V, For 50mL

  • Item #: 10047757
  • MFR SKU: 786-219

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