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PerkinElmer - MPSU-PTERK-K10K - Alpha SureFire Ultra Multiplex p-ERK 1/2 (Eu) + Total ERK 545 (Tb) Kit, 10, 000 Assay Points

PerkinElmer - MPSU-PTERK-K10K - Alpha SureFire Ultra Multiplex p-ERK 1/2 (Eu) + Total ERK 545 (Tb) Kit, 10, 000 Assay Points
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10208013
MPSU-PTERK-K10K

PerkinElmer - MPSU-PTERK-K10K - Alpha SureFire Ultra Multiplex p-ERK 1/2 (Eu) + Total ERK 545 (Tb) Kit, 10, 000 Assay Points

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The Alpha SureFire Ultra HV Multiplex p-ERK 1/2 + Total ERK assay kit is used to measure both the phosphorylation (Thr202/Tyr204) and total levels of endogenous “extracellular signal-regulated kinase 1 and 2” (ERK 1/2) in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure ERK 1/2 activation by either recombinant or endogenous receptors, and can be applied to primary cells. The Alpha SureFire Ultra HV Multiplex p-ERK 1/2 + Total ERK assay kit is used to measure both the phosphorylation (Thr202/Tyr204) and total levels of endogenous “extracellular signal-regulated kinase 1 and 2” (ERK 1/2) in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure ERK 1/2 activation by either recombinant or endogenous receptors, and can be applied to primary cells. The 615nm (Eu) signal corresponds to the phosphorylated ERK analysis, and the 545nm (Tb) signal corresponds to the total ERK analysis. This kit has been formulated to provide improved signal:background (i.e. S:B) assay windows, and to perform without interference in the presence of extraneous antibodies. No-wash steps, no separation steps Multiplexing assay Sensitive detection No interference from exogenous antibodies Results in less than 3 hours   In this assay, the Alpha 615 Acceptor bead is directly-conjugated with an antibody to the phosphorylated site on the target protein. The Alpha 545 Acceptor bead is coated with the CaptSure agent, which binds the CaptSure tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody. The antibodies used in this kit are identical to those used in the individual phospho- and total- ERK AlphaLISA SureFire Ultra kits (ALSU-PERK-A and ALSU-TERK-A catalog numbers), although their orientation of bead attachment may vary between kits.  

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Assay Pathway: MAPK Assay Target: ERK1/2 Assay Target Class: Phosphoprotein, Protein Automation Compatible: Yes Detection Method: Alpha Experimental Type: In vitro Molecular Modification: Phosphorylation Product Brand Name: Alpha SureFire Ultra Multiplex, AlphaPlex Shipping Condition: Blue Ice Target Species: Human Unit Size: 10, 000 assay points


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