Toll Free 1.866.636.2487
NEOBITS
Cart
ITEMS IN CART: 0  TOTAL: $0.00


CALL TOLL FREE
1.866.636.2487


PerkinElmer - AS111R - Anti-DNP Alpha Donor Beads, 25 mg

PerkinElmer - AS111R - Anti-DNP Alpha Donor Beads, 25 mg
Image is an illustration, may not reflect the exact product sold.
10207266
AS111R

PerkinElmer - AS111R - Anti-DNP Alpha Donor Beads, 25 mg

Call For Stock
(Shipping delay possible)
$21,951.50
Request Quote

Alpha Donor beads conjugated to anti-DNP antibody. These beads can be used to capture DNP-labeled proteins, peptides, and small molecules. Alpha Donor beads coated with anti-DNP antibody. These beads can be used to capture DNP-labeled proteins and other DNP-labeled biomolecules, and can be used in conjunction with AlphaScreen, AlphaLISA, or AlphaPlex Acceptor beads to create no-wash assays for: Analyte detection assays Antibody detection assays Antibody binding assays Protein-protein interaction assays Protein detection assays Enzymatic assays In a typical Alpha assay, 1 mg of Donor beads is sufficient to run 1, 000-2, 000 wells using a 25 µL reaction volume. Bead concentration can be adjusted for optimal performance. AlphaScreen and AlphaLISA are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym "Alpha" stands for amplified luminescent proximity homogeneous assay. As the name implies, some of the key features of these technologies are that they are non-radioactive, homogeneous proximity assays. Binding of molecules captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent/fluorescent signal. To understand how a signal is produced, one must begin with an understanding of the beads. AlphaScreen and AlphaLISA assays require two bead types: Donor beads and Acceptor beads. Each bead type contains a different proprietary mixture of chemicals, which are key elements of the AlphaScreen technology. Donor beads contain a photosensitizer, phthalocyanine, which converts ambient oxygen to an excited and reactive form of O2, singlet oxygen, upon illumination at 680 nm. Please note that singlet oxygen is not a radical; it is molecular oxygen with a single excited electron. Like other excited molecules, singlet oxygen has a limited lifetime prior to falling back to ground state. Within its 4 µsec half-life, singlet oxygen can diffuse approximately 200 nm in solution. If an Acceptor bead is within that proximity, energy is transferred from the singlet oxygen to thioxene derivatives within the Acceptor bead, subsequently culminating in light production at 520-620 nm (AlphaScreen) or at 615 nm (AlphaLISA). In the absence of an Acceptor bead, singlet oxygen falls to ground state and no signal is produced. This proximity-dependent chemical energy transfer is the basis for AlphaScreen's homogeneous nature.

See other products in some related categories (click to browse):
Antibody Conjugates: Anti-DNP Automation Compatible: Yes Bead Type or Core Bead Type: Alpha Donor Detection Method: Alpha Experimental Type: In vitro Product Brand Name: Alpha Shipping Condition: Blue Ice Unit Size: 25 mg


Neobits.com is where business goes shopping. With over 5 million products from 10,000+ manufacturers, we offer the largest B2B catalog on the internet (to our knowledge). We cater to the needs of all major verticals, small to large businesses, government, education and healthcare markets, as well as consumers. Through our extensive, carefully developed supplier and logistics network we have instant access to over $100 billion worth of inventory in hundreds of stocking warehouses nationwide. We ship to every state and to nearly 100 countries worldwide.

Our categories include Industrial & Scientific, MRO Products & Supplies, Laboratory & Science, Electronic Components, Occupational Health & Safety, Electronics, Computer & Photo, Access Control & Security, Wireless Carrier Infrastructure, Test & Maintenance, and thousands more. That said, the best way to find products on our website is via search. We are always expanding our catalog, with thousands of new products added daily. If you need something you don't see here, email us at , or call toll free 1.866.636.2487. Prospective new suppliers, please email .

We can send formal quotes or bids upon request. Please submit this form to request a quote. We often have volume discounts, government & education discounts and loyalty discounts. We accept purchase orders from government and public education entities, as well as publicly traded corporations. CAGE Code: 3P0S1.

We are continually investing in people and systems to offer excellent customer service. See some customer testimonials here. You can reach our Customer Care team via email at , or call toll free 1.866.636.2487. The best way to request a return or order cancellation is to submit this form.

Also see: Categories, Products, Brands and Top Searches.


Creditcards Logos
Credit Card Processing
Acceptance Mark View Neobits, Inc. profile on Ariba Discovery