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PerkinElmer - AL552HV - IGF-1 (rat) AlphaLISA Detection Kit, 100 Assay Points

PerkinElmer - AL552HV - IGF-1 (rat) AlphaLISA Detection Kit, 100 Assay Points
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10206780
AL552HV

PerkinElmer - AL552HV - IGF-1 (rat) AlphaLISA Detection Kit, 100 Assay Points

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$711.50

The AlphaLISA immunoassay kit for rat IGF-1 enables the quantitative determination of rat insulin-like growth factor 1 (IGF-1) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). Formats: Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample). Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample). Our 5, 000 assay point kit allows you to run 5, 000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample). Features: No-wash steps, no separation steps ELISA alternative technology Sensitive detection Broad sample compatibility Small sample volume Results in less than 3 hours Half the time of an ELISA assay AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. IGF-1 is a small (70 amino acids) protein with significant resemblance to insulin. However, it is not an analog of insulin in function. It acts as a growth factor during fetal and child development, while acting as an anabolic agent in adults. Abnormal IGF-1 expression can lead to dwarfism, acromegaly, some forms of diabetes and several types of cancer. Some evidence suggests a role in neurological disorders such as ALS. This kit detects rat IGF-1 in serum, plasma and cell culture supernatants.

See other products in some related categories (click to browse):
Assay Target: IGF1 Assay Target Class: Protein Automation Compatible: Yes Detection Method: Alpha Experimental Type: In vitro Product Brand Name: AlphaLISA Shipping Condition: Blue Ice Target Species: Rat Therapeutic Area: Metabolic Unit Size: 100 Assay Points


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