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PerkinElmer - AL516F - CCL5/RANTES (mouse) AlphaLISA Detection Kit, 5, 000 Assay Points

PerkinElmer - AL516F - CCL5/RANTES (mouse) AlphaLISA Detection Kit, 5, 000 Assay Points
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10206632
AL516F

PerkinElmer - AL516F - CCL5/RANTES (mouse) AlphaLISA Detection Kit, 5, 000 Assay Points

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The AlphaLISA Mouse C-C Motif Chemokine 5 / Regulated Upon Activation Normal T-Cell Expressed, and Secreted (CCL5/RANTES) Detection Kit is designed for detection and quantitation of mouse CCL5/RANTES in serum, bronchial lavage fluid (BALF), buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. Formats: Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample). Our 5, 000 assay point kit allows you to run 5, 000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample). Features: No-wash steps, no separation steps ELISA alternative technology Sensitive detection Broad sample compatibility Small sample volume Results in less than 3 hours Half the time of an ELISA assay Mouse C-C Motif Chemokine 5 (CCL5), also known as Regulated upon Activation, Normal T cell Expressed and Secreted (RANTES), is an 8 kDa protein that plays a primary role in the inflammatory immune response. Human CCL5 binds to C-C chemokine receptor (CCR) types 1, 3, and 5. CCL5 dimerization and oligomerization, as well as binding to glycosaminoglycan, are key elements of cell recruitment in the inflammation process. CCL5 is a potent chemoattractant for a number of different cell types, particularly eosinophils, basophils, and mononuclear cells. CCL5 is a potential biomarker for several diseases, for example, it can be useful for indicating the magnitude of asthmatic airway inflammation. In addition to being an important biomarker, CCL5 is studied for its interaction with its receptor, CCR5, in HIV research. AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

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Assay Target: CCL5 Assay Target Class: Cytokine Automation Compatible: Yes Detection Method: Alpha Experimental Type: In vitro Product Brand Name: AlphaLISA Shipping Condition: Blue Ice Target Species: Mouse Therapeutic Area: Inflammation Unit Size: 5, 000 assay points


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