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ITEM #: 11575344 The Guava Auto CD4/CD4% Kit for Flow Cytometry is a two-color direct immunofluorescence reagent kit for enumeration of mature CD4+ T lymphocytes & the determination of CD4 T cells as a percent of total lymphocytes in human blood.
Overview The Guava® Auto CD4/CD4% Kit is a two-color direct immunofluorescence reagent kit for enumeration of mature CD4+ T lymphocytes and the determination of CD4 T cells as a percent of total lymphocytes in human blood. The kit consists of the antibody cocktail, a proprietary mixture of anti-human lymphocyte antibodies conjugated to the tandem dye phycoerythrin (PE)-Cy5 (PECy5) and a monoclonal anti-human CD4 antibody conjugated to PE, and Guava® 1X Lysing Solution to lyse erythrocytes. The anti-human lymphocyte antibodies detect all human lymphocytes. The CD4 antibody allows the identification of human helper/inducer CD4+ T cell (HLA Class II reactive) and recognizes a 60, 000 Da surface antigen. Monocytes also express CD4 but at lower density, and have no co-expression of the other lymphocyte markers detected by reagents in this kit. The Auto CD4/CD4% Kit should be used only with the Guava® Auto CD4/CD4% Software Module, which allows for automatic acquisition and gating and provides test results. Components
Five 1-mL vials of Guava Auto CD4/CD4% Antibody Cocktail (Part Number 4700-1160)
Five 40-mL bottles of Guava 1X Lysing Solution (Part Number 4700-0082)
Detection method Fluorescent
Format Phycoerythrin/Cy5 Application The Guava Auto CD4/CD4% Kit for Flow Cytometry is a two-color direct immunofluorescence reagent kit for enumeration of mature CD4+ T lymphocytes & the determination of CD4 T cells as a percent of total lymphocytes in human blood.
Key Applications
Flow Cytometry
Species Reactivity
Human
Cell Number per Assay 10 μ L whole blood
Sample Format
Tube
Sample Volume 400 µ L Emission max.
575 nm
Excitation max.
532 nm
Sales Restrictions This product is restricted for sale in United States, European Union, Japan, and certain other regulated countries.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
