Overview The InnoCyte™ Monoctye Cell Migration Assay is designed to study the effects of various drugs or agents on monocyte motility or for identifying chemoattractant molecules. The assay is not intended for adherent cell lines, such as fibroblasts or solid tumor cell lines.
Catalogue Number CBA098
Brand Family Calbiochem®
Materials Required but Not Delivered • Fluorescence plate reader capable of measuring fluorescence in 96-well plates at an excitation wavelength of ~485 nm and an emission wavelength of ~520 nm
• Pipettes
• PBS for washing the cells
References Cravens, P.D. 2007. Scan. J. Immunol. 65, 514.
Charo, I.F., et al. 2006. N. Engl. J. Med. 354, 610.
Knitscher, U., et al. 2000. Eur. J. Pharmacol. 401, 259.
Turner, L., et al. 1999. Eur. J. Immunol. 29, 2288.
Cross, A.K., et al. 1997. Cytokine 9, 521.
Detection method Fluorometric
Form 96 Tests
Format 96-Well Plate
Kit contains 96-Well Monocyte Cell Migration Chamber, Black, Conical 96-Well Plate, D-PBS, Calcein-AM, and a user protocol.
Quality Level MQ100
Sample Type Monocytes
R Phrase R: 36/37/38
Irritating to eyes, respiratory system and skin.
S Phrase S: 26-36
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Intended use The Calbiochem ® InnoCyte™ 96-Well Monoctye Cell Migration Assay is intended for the study of the effects of various drugs or agents on monocyte motility or for identifying chemoattractant molecules. The assay is not intended for adherent cell lines, such as fibroblasts or solid tumor cell lines. To study these cell types, please use Cat. No. CBA010 or CBA017.
Ship Code Multiple Storage Conditions
Toxicity Irritant
Storage Multiple storage requirements
Storage Conditions Upon arrival store the Microplates at room temperature, the Calcein-AM at -20° C and the remaining components at 4° C.
Do not freeze Ok to freeze
Kit contains 96-Well Monocyte Cell Migration Chamber, Black, Conical 96-Well Plate, D-PBS, Calcein-AM, and a user protocol.
